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What is free testosterone? A plain-language definition

Free testosterone is the portion of the hormone in your blood that is not attached to a protein. It is a small share of the total, and it is measured in some situations where the total number could mislead.

Free and total testosterone

Free and total testosterone on one order, so you get both numbers from one draw.

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Free means unbound

MedlinePlus explains that all the testosterone in your blood is either bound to proteins or unbound. The main binding proteins are sex hormone-binding globulin (SHBG) and albumin. Most testosterone is bound. The Endocrine Society guideline puts the unbound share at about 2 to 4 percent.

Bound testosterone is not as easily used by the body. MedlinePlus adds that having most of it bound is necessary for health, because it keeps the body from using too much.

Why anyone measures it

A total testosterone test counts both bound and unbound hormone. When SHBG is higher or lower than usual, the total number can give a misleading picture. Checking free testosterone helps in those cases.

The Endocrine Society guideline recommends it when total testosterone is at or near the lower limit of normal, or when a condition alters SHBG, such as obesity, diabetes, hypothyroidism, aging, or liver disease such as cirrhosis. See free vs total testosterone for the full list and the trade-offs.

How it is measured

Free testosterone can be measured directly by equilibrium dialysis, or calculated from total testosterone, SHBG and albumin. The guideline accepts calculating free testosterone by formula when equilibrium dialysis is not available.

Getting a free testosterone figure

If you want a free testosterone figure, the free and total test gives both numbers on one order. If you already have total and SHBG results, the free testosterone calculator uses a published formula to estimate free testosterone. It assumes a typical albumin level unless you enter your own, and gives an estimate, not a lab result.

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What it can’t tell you

Free testosterone is one number. It can’t diagnose a condition by itself, and the guideline says no harmonized reference range exists for it, so ranges depend on the method and the lab. See free testosterone levels.

If you have symptoms such as low sex drive or erectile dysfunction, see a clinician whatever your number is.

Free and total testosterone

$99

See this test at HealthLabs

Price checked October 2026. Not available in ND, RI, NY, NJ, MD and HI. We earn a commission on sales.

Keep reading

Sources

  1. Testosterone Levels Test (MedlinePlus)
  2. Testosterone Therapy in Men With Hypogonadism: An Endocrine Society Clinical Practice Guideline (2018)